Breast cancer proteomics reveals correlation between estrogen receptor status and differential phosphorylation of PGRMC1.

ABSTRACT:

Introduction

Breast tumors lacking the estrogen receptor-alpha (ER-alpha) have increased incidence of resistance to therapy and poorer clinical prognosis.

Methods

Whole tissue sections from 16 cryopreserved breast cancer tumors that were either positive or negative for the ER (eight ER positive and eight ER negative) were differentially analyzed by multiplex imaging of two-dimensional PAGE gels using 54 cm isoelectric focusing. Differentially detected spots of Progesterone Receptor Membrane Component 1 (PGRMC1) were shown to differ in phosphorylation status by differential two dimensional polyacrylamide gel electrophoresis of phosphatase-treated tumor proteins. Site directed mutagenesis was used to create putative phosphorylation site point mutants in PGRMC1. Stable transfec

SUBMITTER: Neubauer H 

PROVIDER: S-EPMC2614521 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature