In vitro selection of a neutralization-resistant hepatitis C virus escape mutant.

ABSTRACT: Effective immunization against hepatitis C virus (HCV) infections is likely to require the induction of both robust T and B cell immunity. Although neutralizing antibodies may play an important role in control of infection, there is little understanding of the structure of the HCV envelope glycoproteins and how they interact with such antibodies. An additional challenge for vaccine design is the genetic diversity of HCV and the rapid evolution of viral quasispecies that escape antibody-mediated neutralization. We used a cell culture-infectious, chimeric HCV with the structural proteins of genotype 1a virus to identify envelope residues contributing to the epitope recognized by a broadly neutralizing, murine monoclonal antibody, AP33. By repetitive rounds of neutralization followed by ampli

SUBMITTER: Gal-Tanamy M 

PROVIDER: S-EPMC2614781 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature