A tip-localized RhoGAP controls cell polarity by globally inhibiting Rho GTPase at the cell apex.

ABSTRACT:

Background

Highly elongated eukaryotic cells (e.g., neuronal axons, fungal hyphae, and pollen tubes) are generated through continuous apically restricted growth (tip growth), which universally requires tip-localized Rho GTPases. We used the oscillating pollen tube as a model system to determine the function and regulation of Rho GTPases in tip growth. Our previous work showed that the spatiotemporal dynamics of the apical cap of the activated Rho-like GTPase from Plant 1 (ROP1) are critical for tip growth in pollen tubes. However, the underlying mechanism for the generation and maintenance of this dynamic apical cap is poorly understood.

Results

A screen for mutations that enhance ROP1-overexpression-induced depolarization of pollen-tube growth identified REN1 (ROP1 enhancer

SUBMITTER: Hwang JU 

PROVIDER: S-EPMC2615002 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature