"Proteotyping": population proteomics of human leukocytes using top down mass spectrometry.

ABSTRACT: Characterizing combinations of coding polymorphisms (cSNPs), alternative splicing and post-translational modifications (PTMs) on a single protein by standard peptide-based proteomics is challenging owing to <100% sequence coverage and the uncoupling effect of proteolysis on such variations >10-20 residues apart. Because top down MS measures the whole protein, combinations of all the variations affecting primary sequence can be detected as they occur in combination. The protein form generated by all types of variation is here termed the "proteotype", akin to a haplotype at the DNA level. Analysis of proteins from human primary leukocytes harvested from leukoreduction filters using a dual on-line/off-line top down MS strategy produced >600 unique intact masses, 133 of which were identified f

SUBMITTER: Roth MJ 

PROVIDER: S-EPMC2615201 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature