The expression of adiponectin receptors and the effects of adiponectin and leptin on airway smooth muscle cells.

ABSTRACT:

Purpose

Obesity is a major risk factor for asthma and it influences airway smooth muscle function and responsiveness. Adiponectin is inversely associated with obesity and its action is mediated through at least 2 cell membrane receptors (AdipoR1 and AdipoR2). Leptin is positively associated with obesity. We investigated whether human airway smooth muscle (ASM) cells express adiponectin receptors and whether adiponectin and leptin regulate human ASM cell proliferation and vascular endothelial growth factor (VEGF) release.

Materials and methods

Human ASM cells were growth- arrested in serum-deprived medium for 48 hours and then stimulated with PDGF, adiponectin and leptin. After 48 hours of stimulation, proliferation was determined using a cell proliferation ELISA kit. Human A

SUBMITTER: Shin JH 

PROVIDER: S-EPMC2615381 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature