Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium.

ABSTRACT:

Background

Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase (MMP-1) are considered to play important roles in this process. We examined MMP-2 and -9 expression using zymography, in situ hybridization, real-time PCR, and microarray analysis to clarify their roles in the bovine endometrium during gestation.

Methods

Endometria, placentomes, and fetal membranes were collected from Japanese black cows that were killed on day 15 to 252 of gestation or during their estrous cycle. The gene expression of MMP-related molecules (mainly MMP-2 and -9)

SUBMITTER: Kizaki K 

PROVIDER: S-EPMC2615774 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature