Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.

ABSTRACT: We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

SUBMITTER: Demaneche S 

PROVIDER: S-EPMC2620714 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature