Two Kdo-heptose regions identified in Hafnia alvei 32 lipopolysaccharide: the complete core structure and serological screening of different Hafnia O serotypes.

ABSTRACT: Hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. Various 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo)-containing fragments different from known structures of core oligosaccharides were previously found among fractions obtained by mild acid hydrolysis of some H. alvei lipopolysaccharides (LPSs). However, the positions of these segments in the LPS structure were not known. Analysis of de-N,O-acylated LPS by nuclear magnetic resonance spectroscopy and mass spectrometry allowed the determination of the location of a Kdo-containing trisaccharide in the structure of H. alvei PCM 32 LPS. It was established that the trisaccharide {L-alpha-D-Hepp-(1-->4)-[alpha-D-Galp6OAc-(1-->7)]-alpha-Kd

SUBMITTER: Lukasiewicz J 

PROVIDER: S-EPMC2620813 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature