High throughput protein-protein interaction data: clues for the architecture of protein complexes.

ABSTRACT:

Background

High-throughput techniques are becoming widely used to study protein-protein interactions and protein complexes on a proteome-wide scale. Here we have explored the potential of these techniques to accurately determine the constituent proteins of complexes and their architecture within the complex.

Results

Two-dimensional representations of the 19S and 20S proteasome, mediator, and SAGA complexes were generated and overlaid with high quality pairwise interaction data, core-module-attachment classifications from affinity purifications of complexes and predicted domain-domain interactions. Pairwise interaction data could accurately determine the members of each complex, but was unexpectedly poor at deciphering the topology of proteins in complexes. Core and module da

SUBMITTER: Krycer JR 

PROVIDER: S-EPMC2621150 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature