Evaluating genome-wide DNA methylation changes in mice by Methylation Specific Digital Karyotyping.

ABSTRACT:

Background

The study of genome-wide DNA methylation changes has become more accessible with the development of various array-based technologies though when studying species other than human the choice of applications are limited and not always within reach. In this study, we adapted and tested the applicability of Methylation Specific Digital Karyotyping (MSDK), a non-array based method, for the prospective analysis of epigenetic changes after perinatal nutritional modifications in a mouse model of allergic airway disease. MSDK is a sequenced based method that allows a comprehensive and unbiased methylation profiling. The method generates 21 base pairs long sequence tags derived from specific locations in the genome. The resulting tag frequencies determine in a quantitative manner

SUBMITTER: Boon K 

PROVIDER: S-EPMC2621211 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature