Multiplex immunoassay analysis of biomarkers in clinically accessible quantities of human aqueous humor.

ABSTRACT:

Purpose

Aqueous humor is intimately related to the cells of the anterior and posterior chambers, which affect its composition. Aqueous analysis provides useful information regarding physiological and pathophysiological processes in the eye. Human aqueous samples are typically less than 100 microl, limiting the usefulness of the analysis with traditional Enzyme-Linked immunoSorbant Assay (ELISA) techniques. The specific aim of this study was to investigate if whether large numbers of analytes can be identified in clinically available samples of aqueous humor and to document the detectability of certain biomarkers in the aqueous.

Methods

We used a technology developed by Luminex xMAP to analyze hundreds of analytes in a small sample. Aqueous from eight normal and two diabetic

SUBMITTER: Sharma RK 

PROVIDER: S-EPMC2622713 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature