Comparison of a label-free quantitative proteomic method based on peptide ion current area to the isotope coded affinity tag method.

ABSTRACT: Recently, several research groups have published methods for the determination of proteomic expression profiling by mass spectrometry without the use of exogenously added stable isotopes or stable isotope dilution theory. These so-called label-free, methods have the advantage of allowing data on each sample to be acquired independently from all other samples to which they can later be compared in silico for the purpose of measuring changes in protein expression between various biological states. We developed label free software based on direct measurement of peptide ion current area (PICA) and compared it to two other methods, a simpler label free method known as spectral counting and the isotope coded affinity tag (ICAT) method. Data analysis by these methods of a standard mixture contain

SUBMITTER: Ryu S 

PROVIDER: S-EPMC2623286 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature