High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies.

ABSTRACT:

Background

Massively parallel DNA sequencing instruments are enabling the decoding of whole genomes at significantly lower cost and higher throughput than classical Sanger technology. Each of these technologies have been estimated to yield assemblies with more problematic features than the standard method. These problems are of a different nature depending on the techniques used. So, an appropriate mix of technologies may help resolve most difficulties, and eventually provide assemblies of high quality without requiring any Sanger-based input.

Results

We compared assemblies obtained using Sanger data with those from different inputs from New Sequencing Technologies. The assemblies were systematically compared with a reference finished sequence. We found that the 454 GSFLX ca

SUBMITTER: Aury JM 

PROVIDER: S-EPMC2625371 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature