The Aspergillus nidulans kinesin-3 UncA motor moves vesicles along a subpopulation of microtubules.

ABSTRACT: The extremely polarized growth form of filamentous fungi imposes a huge challenge on the cellular transport machinery, because proteins and lipids required for hyphal extension need to be continuously transported to the growing tip. Recently, it was shown that endocytosis is also important for hyphal growth. Here, we found that the Aspergillus nidulans kinesin-3 motor protein UncA transports vesicles and is required for fast hyphal extension. Most surprisingly, UncA-dependent vesicle movement occurred along a subpopulation of microtubules. Green fluorescent protein (GFP)-labeled UncA(rigor) decorated a single microtubule, which remained intact during mitosis, whereas other cytoplasmic microtubules were depolymerized. Mitotic spindles were not labeled with GFP-UncA(rigor) but reacted with a

SUBMITTER: Zekert N 

PROVIDER: S-EPMC2626562 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature