The use of amphipols as universal molecular adapters to immobilize membrane proteins onto solid supports.

ABSTRACT: Because of the importance of their physiological functions, cell membranes represent critical targets in biological research. Membrane proteins, which make up approximately 1/3 of the proteome, interact with a wide range of small ligands and macromolecular partners as well as with foreign molecules such as synthetic drugs, antibodies, toxins, or surface recognition proteins of pathogenic organisms. Whether it is for the sake of basic biomedical or pharmacological research, it is of great interest to develop tools facilitating the study of these interactions. Surface-based in vitro assays are appealing because they require minimum quantities of reagents, and they are suitable for multiplexing and high-throughput screening. We introduce here a general method for immobilizing functional, unmo

SUBMITTER: Charvolin D 

PROVIDER: S-EPMC2626715 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature