Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.

ABSTRACT: The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process.

SUBMITTER: Kagawa M 

PROVIDER: S-EPMC262715 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature