Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes.

ABSTRACT: RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), the causal agent of a worldwide seed-borne, aphid-transmitted viral soybean disease, we obtained all full-length genome sequences of SMV as well as partial sequences encoding the N-terminal most (P1 protease) and the C-terminal most (capsid protein; CP) viral protein. The sequences were analyzed for possible recombination events using a variety of automatic and manual recombination detection and verification approaches. Automatic scanning identified 3, 10, and 17 recombination sites in the P1, CP

SUBMITTER: Gagarinova AG 

PROVIDER: S-EPMC2627826 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature