Transgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts.

ABSTRACT:

Background

Seed oil accumulates primarily as triacylglycerol (TAG). While the biochemical pathway for TAG biosynthesis is known, its regulation remains unclear. Previous research identified microsomal diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) as controlling a rate-limiting step in the TAG biosynthesis pathway. Of note, overexpression of DGAT1 results in substantial increases in oil content and seed size. To further analyze the global consequences of manipulating DGAT1 levels during seed development, a concerted transcriptome and metabolome analysis of transgenic B. napus prototypes was performed.

Results

Using a targeted Brassica cDNA microarray, about 200 genes were differentially expressed in two independent transgenic lines analyzed. Interestingly, 24-33% of t

SUBMITTER: Sharma N 

PROVIDER: S-EPMC2627897 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature