Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair.

ABSTRACT:

Background

Neisseria meningitidis, the causative agent of meningococcal disease, is exposed to high levels of reactive oxygen species inside its exclusive human host. The DNA glycosylase Fpg of the base excision repair pathway (BER) is a central player in the correction of oxidative DNA damage. This study aimed at characterizing the meningococcal Fpg and its role in DNA repair.

Results

The deduced N. meningitidis Fpg amino acid sequence was highly homologous to other Fpg orthologues, with particularly high conservation of functional domains. As for most N. meningitidis DNA repair genes, the fpg gene contained a DNA uptake sequence mediating efficient transformation of DNA. The recombinant N. meningitidis Fpg protein was over-expressed, purified to homogeneity and assessed fo

SUBMITTER: Tibballs KL 

PROVIDER: S-EPMC2628661 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature