Inhibitors of melanogenesis increase toxicity of cyclophosphamide and lymphocytes against melanoma cells.

ABSTRACT: High mortality rate for metastatic melanoma is related to its resistant to the current methods of therapy. Melanogenesis is a metabolic pathway characteristic for normal and malignant melanocytes that can affect the behavior of melanoma cells or its surrounding environment. Human melanoma cells in which production of melanin pigment is dependent on tyrosine levels in medium were used for experiments. Peripheral blood mononuclear cells were derived from the buffy coats purchased from Lifeblood Biological Services. Cell pigmentation was evaluated macroscopically, and tyrosinase activity was measured spectrophotometrically. Cell proliferation and viability were measured using lactate dehydrogenase release MTT, [(3)H]-thymidine incorporation and DNA content analyses, and gene expression was me

SUBMITTER: Slominski A 

PROVIDER: S-EPMC2628959 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature