Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions.

ABSTRACT:

Background

Quantitative data from gene expression experiments are often normalized by transcription levels of reference or housekeeping genes. An inherent assumption for their use is that the expression of these genes is highly uniform in living organisms during various phases of development, in different cell types and under diverse environmental conditions. To date, the validation of reference genes in plants has received very little attention and suitable reference genes have not been defined for a great number of crop species including Coffea arabica. The aim of the research reported herein was to compare the relative expression of a set of potential reference genes across different types of tissue/organ samples of coffee. We also validated the expression profiles of the select

SUBMITTER: Barsalobres-Cavallari CF 

PROVIDER: S-EPMC2629470 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature