Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process.

ABSTRACT:

Background

The elucidation of gene expression patterns leads to a better understanding of biological processes. Real-time quantitative RT-PCR has become the standard method for in-depth studies of gene expression. A biologically meaningful reporting of target mRNA quantities requires accurate and reliable normalization in order to identify real gene-specific variation. The purpose of normalization is to control several variables such as different amounts and quality of starting material, variable enzymatic efficiencies of retrotranscription from RNA to cDNA, or differences between tissues or cells in overall transcriptional activity. The validity of a housekeeping gene as endogenous control relies on the stability of its expression level across the sample panel being analysed. In t

SUBMITTER: Exposito-Rodriguez M 

PROVIDER: S-EPMC2629474 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature