Development and evaluation of a real-time one step reverse-transcriptase PCR for quantitation of Chandipura virus.

ABSTRACT:

Background

Chandipura virus (CHPV), a member of family Rhabdoviridae was attributed to an explosive outbreak of acute encephalitis in children in Andhra Pradesh, India in 2003 and a small outbreak among tribal children from Gujarat, Western India in 2004. The case-fatality rate ranged from 55-75%. Considering the rapid progression of the disease and high mortality, a highly sensitive method for quantifying CHPV RNA by real-time one step reverse transcriptase PCR (real-time one step RT-PCR) using TaqMan technology was developed for rapid diagnosis.

Methods

Primers and probe for P gene were designed and used to standardize real-time one step RT-PCR assay for CHPV RNA quantitation. Standard RNA was prepared by PCR amplification, TA cloning and run off transcription. The optimiz

SUBMITTER: Kumar S 

PROVIDER: S-EPMC2629476 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature