Evaluation of MFRP as a candidate gene for high hyperopia.

ABSTRACT:

Purpose

Mutations in the membrane-type frizzled-related protein (MFRP) gene have been identified in patients with pathologic high hyperopia associated with nanophthalmos or microphthalmia. This study is to test if a mutation in MFRP is responsible for physiologic high hyperopia.

Methods

DNA was prepared from venous leukocytes of 51 patients with physiologic high hyperopia (refraction of spherical equivalent > or = +5.00 [diopters] D) and 96 controls (refraction of spherical equivalent between -0.50 D and +1.00 D). The coding regions and adjacent intronic sequence of MFRP were amplified by polymerase chain reaction (PCR) and were then analyzed by cycle sequencing. Variations detected were further evaluated in normal controls and available family members by heteroduplex- singl

SUBMITTER: Wang P 

PROVIDER: S-EPMC2629737 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature