An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.

ABSTRACT:

Background

Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important for mechanistic and functional studies. Although many site-directed mutagenesis methods have been developed, a simple, quick and multi-applicable method is still desirable.

Results

We have developed a site-directed plasmid mutagenesis protocol that preserved the simple one step procedure of the QuikChange site-directed mutagenesis but enhanced its efficiency and extended its capability for multi-site mutagenesis. This modified protocol used a new primer design that promoted primer

SUBMITTER: Liu H 

PROVIDER: S-EPMC2629768 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature