A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering.

ABSTRACT:

Background

The nematode Caenorhabditis elegans has emerged as a powerful system to study biologic questions ranging from development to aging. The generation of transgenic animals is an important experimental tool and allows use of GFP fusion proteins to study the expression of genes of interest or generation of epitope tagged versions of specific genes. Transgenes are often generated by placing a promoter upstream of a reporter gene or cDNA. This often produces a representative expression pattern, but important exceptions have been observed. To better capture the genuine expression pattern and timing, several investigators have modified large pieces of DNA carried by BACs or fosmids for use in the construction of transgenic animals via recombineering. However, these techniques are

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC2629773 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature