Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots.

ABSTRACT:

Background

White lupin (Lupinus albus L.) roots efficiently take up and accumulate (heavy) metals, adapt to phosphate deficiency by forming cluster roots, and secrete antimicrobial prenylated isoflavones during development. Genomic and proteomic approaches were applied to identify candidate genes and proteins involved in antimicrobial defense and (heavy) metal uptake and translocation.

Results

A cDNA library was constructed from roots of white lupin seedlings. Eight thousand clones were randomly sequenced and assembled into 2,455 unigenes, which were annotated based on homologous matches in the NCBInr protein database. A reference map of developing white lupin root proteins was established through 2-D gel electrophoresis and peptide mass fingerprinting. High quality peptide

SUBMITTER: Tian L 

PROVIDER: S-EPMC2630931 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature