Single-chain Fv phage display propensity exhibits strong positive correlation with overall expression levels.

ABSTRACT:

Background

Single chain Fvs (scFvs) are widely applied in research, diagnostics and therapeutic settings. Display and selection from combinatorial libraries is the main route to their discovery and many factors influence the success of this process. They exhibit low thermodynamic stability, resulting in low levels of premature cytosolic folding or aggregation which facilitates sec YEG-mediated translocation and phage in E. coli. However, there is little data analysing how this is related to and influenced by scFv protein expression.

Results

We characterised the relationship between overall scFv expression and display propensity for a panel of 15 anti-tetanus toxin scFvs and found a strong positive correlation (Rho = 0.88, p < 0.005) between the two parameters. Display propen

SUBMITTER: Scott N 

PROVIDER: S-EPMC2630973 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature