Ligand-induced conformational changes in a thermophilic ribose-binding protein.

ABSTRACT:

Background

Members of the periplasmic binding protein (PBP) superfamily are involved in transport and signaling processes in both prokaryotes and eukaryotes. Biological responses are typically mediated by ligand-induced conformational changes in which the binding event is coupled to a hinge-bending motion that brings together two domains in a closed form. In all PBP-mediated biological processes, downstream partners recognize the closed form of the protein. This motion has also been exploited in protein engineering experiments to construct biosensors that transduce ligand binding to a variety of physical signals. Understanding the mechanistic details of PBP conformational changes, both global (hinge bending, twisting, shear movements) and local (rotamer changes, backbone motion), t

SUBMITTER: Cuneo MJ 

PROVIDER: S-EPMC2630998 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature