Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil.

ABSTRACT:

Background

Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease that affects cloven-hoofed domestic and wild animals. Virus isolation and enzyme-linked immunosorbent assay (ELISA) are the gold standard tests for diagnosis of FMD. As these methods are time consuming, assays based on viral nucleic acid amplification have been developed.

Results

A previously described real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay with high sensitivity and specificity under laboratorial and experimental conditions was used in the current study. To verify the applicability of this assay under field conditions in Brazil, 460 oral swabs from cattle were collected in areas free of FMD (n = 200) and from areas with outbreaks of FMD (

SUBMITTER: Paixao TA 

PROVIDER: S-EPMC2631516 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature