Ski regulates muscle terminal differentiation by transcriptional activation of Myog in a complex with Six1 and Eya3.

ABSTRACT: Overexpression of the Ski pro-oncogene has been shown to induce myogenesis in non-muscle cells, to promote muscle hypertrophy in postnatal mice, and to activate transcription of muscle-specific genes. However, the precise role of Ski in muscle cell differentiation and its underlying molecular mechanism are not fully understood. To elucidate the involvement of Ski in muscle terminal differentiation, two retroviral systems were used to achieve conditional overexpression or knockdown of Ski in satellite cell-derived C2C12 myoblasts. We found that enforced expression of Ski promoted differentiation, whereas loss of Ski severely impaired it. Compromised terminal differentiation in the absence of Ski was likely because of the failure to induce myogenin (Myog) and p21 despite normal expression of

SUBMITTER: Zhang H 

PROVIDER: S-EPMC2631951 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature