Genomic selection of reference genes for real-time PCR in human myocardium.

ABSTRACT:

Background

Reliability of real-time PCR (RT-qPCR) data is dependent on the use of appropriate reference gene(s) for normalization. To date, no validated reference genes have been reported for normalizing gene expression in human myocardium. This study aimed to identify validated reference genes for use in gene expression studies of failed and non-failed human myocardium.

Methods

Bioinformatic analysis of published human heart gene expression arrays (195 failed hearts, 16 donor hearts) was used to identify 10 stable and abundant genes for further testing. The expression stability of these genes was investigated in 28 failed and 28 non-failed human myocardium samples by RT-qPCR using geNorm software.

Results

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SUBMITTER: Pilbrow AP 

PROVIDER: S-EPMC2632664 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature