Endothelin-1 impairs alveolar epithelial function via endothelial ETB receptor.

ABSTRACT:

Rationale

Endothelin-1 (ET-1) is increased in patients with high-altitude pulmonary edema and acute respiratory distress syndrome, and these patients have decreased alveolar fluid reabsorption (AFR).

Objectives

To determine whether ET-1 impairs AFR via activation of endothelial cells and nitric oxide (NO) generation.

Methods

Isolated perfused rat lung, transgenic rats deficient in ETB receptors, coincubation of lung human microvascular endothelial cells (HMVEC-L) with rat alveolar epithelial type II cells or A549 cells, ouabain-sensitive 86Rb+ uptake.

Measurements and main results

The ET-1-induced decrease in AFR was prevented by blocking the endothelin receptor ETB, but not ETA. Endothelial-epithelial cell interaction is required, as direct exposure of alveola

SUBMITTER: Comellas AP 

PROVIDER: S-EPMC2633058 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature