Transcriptome-guided characterization of genomic rearrangements in a breast cancer cell line.
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ABSTRACT:
SUBMITTER: Zhao Q
PROVIDER: S-EPMC2633215 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
Transcriptome-guided characterization of genomic rearrangements in a breast cancer cell line.
Zhao Qi Q Caballero Otavia L OL Levy Samuel S Stevenson Brian J BJ Iseli Christian C de Souza Sandro J SJ Galante Pedro A PA Busam Dana D Leversha Margaret A MA Chadalavada Kalyani K Rogers Yu-Hui YH Venter J Craig JC Simpson Andrew J G AJ Strausberg Robert L RL
Proceedings of the National Academy of Sciences of the United States of America 20090130 6
We have identified new genomic alterations in the breast cancer cell line HCC1954, using high-throughput transcriptome sequencing. With 120 Mb of cDNA sequences, we were able to identify genomic rearrangement events leading to fusions or truncations of genes including MRE11 and NSD1, genes already implicated in oncogenesis, and 7 rearrangements involving other additional genes. This approach demonstrates that high-throughput transcriptome sequencing is an effective strategy for the characterizat ...[more]
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