A universal assay for detection of oncogenic fusion transcripts by oligo microarray analysis.

ABSTRACT:

Background

The ability to detect neoplasia-specific fusion genes is important not only in cancer research, but also increasingly in clinical settings to ensure that correct diagnosis is made and the optimal treatment is chosen. However, the available methodologies to detect such fusions all have their distinct short-comings.

Results

We describe a novel oligonucleotide microarray strategy whereby one can screen for all known oncogenic fusion transcripts in a single experiment. To accomplish this, we combine measurements of chimeric transcript junctions with exon-wise measurements of individual fusion partners. To demonstrate the usefulness of the approach, we designed a DNA microarray containing 68,861 oligonucleotide probes that includes oligos covering all combinations of c

SUBMITTER: Skotheim RI 

PROVIDER: S-EPMC2633275 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature