Combining results from lectin affinity chromatography and glycocapture approaches substantially improves the coverage of the glycoproteome.

ABSTRACT: Identification of glycosylated proteins, especially those in the plasma membrane, has the potential of defining diagnostic biomarkers and therapeutic targets as well as increasing our understanding of changes occurring in the glycoproteome during normal differentiation and disease processes. Although many cellular proteins are glycosylated they are rarely identified by mass spectrometric analysis (e.g. shotgun proteomics) of total cell lysates. Therefore, methods that specifically target glycoproteins are necessary to facilitate their isolation from total cell lysates prior to their identification by mass spectrometry-based analysis. To enrich for plasma membrane glycoproteins the methods must selectively target characteristics associated with proteins within this compartment. We demonstra

SUBMITTER: McDonald CA 

PROVIDER: S-EPMC2634582 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature