Secreted trefoil factor 2 activates the CXCR4 receptor in epithelial and lymphocytic cancer cell lines.

ABSTRACT: The secreted trefoil factor family 2 (TFF2) protein contributes to the protection of the gastrointestinal mucosa from injury by strengthening and stabilizing mucin gels, stimulating epithelial restitution, and restraining the associated inflammation. Although trefoil factors have been shown to activate signaling pathways, no cell surface receptor has been directly linked to trefoil peptide signaling. Here we demonstrate the ability of TFF2 peptide to activate signaling via the CXCR4 chemokine receptor in cancer cell lines. We found that both mouse and human TFF2 proteins (at approximately 0.5 microm) activate Ca2+ signaling in lymphoblastic Jurkat cells that could be abrogated by receptor desensitization (with SDF-1alpha) or pretreatment with the specific antagonist AMD3100 or an anti-CXCR

SUBMITTER: Dubeykovskaya Z 

PROVIDER: S-EPMC2635042 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature