Ontology highlight
ABSTRACT:
SUBMITTER: Jamshad M
PROVIDER: S-EPMC2635529 | biostudies-literature | 2008 Feb
REPOSITORIES: biostudies-literature
Jamshad Mohammed M Rajesh Sundaresan S Stamataki Zania Z McKeating Jane A JA Dafforn Timothy T Overduin Michael M Bill Roslyn M RM
Protein expression and purification 20071024 2
Human CD81 (hCD81) protein has been recombinantly produced in the methylotrophic yeast Pichia pastoris. The purified protein, produced at a yield of 1.75 mg/L of culture, was shown to interact with Hepatitis C virus E2 glycoprotein. Immunofluorescent and flow cytometric staining of P. pastoris protoplasts with monoclonal antibodies specific for the second extracellular loop (EC2) of hCD81 confirmed the antigenicity of the recombinant molecule. Full-length hCD81 was solubilized with an array of d ...[more]