Design, synthesis, and evaluation of biotinylated opioid derivatives as novel probes to study opioid pharmacology.

ABSTRACT: A generally applicable strategy of chemically labeling (-)-morphine (1) is described. The synthesis starts from commercially available starting materials and can be completed in two steps with an overall yield of 23%. In silico simulation and NMR results show that the binding of (-)-morphine to one of its molecular targets, toll-like receptor 4 (TLR4), was not affected by the modification. Secreted embryonic alkaline phosphatase (SEAP) reporter assay results demonstrate that C(3) biotinylated and unmodified (-)-morphine show similar biological activities in live cells. To our knowledge, these studies provide the first practical and concise method to label various opioid derivatives, a group of important therapeutics in pain management, for biochemical/pharmacological studies.

SUBMITTER: Li Y 

PROVIDER: S-EPMC2636689 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature