The hierarchically organized splitting of chromosomal bands for all human chromosomes.

ABSTRACT:

Background

Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.

Results

Here we present for the first time the hierarchically organized splitting of chromosomal bands in their sub-bands for all human chromosomes. To do this, array-proved multicolor banding (aMCB) probe-sets for all human chromosomes were applied to normal metaphase spreads of three different G-band levels. We confirmed for all chromosomes to be a general principle that only Giemsa-dark bands split into dark and light sub-bands, as we demonstrated previously by chromosome stretching. Thus, the biological band splitting is in > 50% of the sub-bands differe

SUBMITTER: Kosyakova N 

PROVIDER: S-EPMC2636822 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature