Dual role of Sp3 transcription factor as an inducer of apoptosis and a marker of tumour aggressiveness.

ABSTRACT:

Background

The ambiguous role of transcription factor Sp3 for tumour progression is still debated since it was described as a transcriptional repressor or activator. Here we tried to decipher the molecular mechanisms implicated in Sp3 accumulation observed in aggressive tumours.

Methodology

We generated normal and tumour cell lines conditionally expressing Sp3. Cell growth was analyzed in vitro and after inoculation in nude mice. Apoptosis was assessed by pan- caspase activity assays, by counting fragmented nuclei and by determination of caspase 9 cleavage. Gene expression was determined by quantitative PCR. Cleavage by different caspases was performed after in vitro translation of the Sp3 cDNA in the presence of [S(35)] labelled methionine. Different tumour cell lines and h

SUBMITTER: Essafi-Benkhadir K 

PROVIDER: S-EPMC2636865 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature