Microarray analyses of gene expression during the Tetrahymena thermophila life cycle.

ABSTRACT:

Background

The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression.

Methodology/principal findings

A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation.

Conclusions/significance

Of the approximately 27,000 predicted open reading frames, transcripts homologous to only approximately 5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over

SUBMITTER: Miao W 

PROVIDER: S-EPMC2636879 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature