Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.

ABSTRACT: Understanding molecular-scale architecture of cells requires determination of 3D locations of specific proteins with accuracy matching their nanometer-length scale. Existing electron and light microscopy techniques are limited either in molecular specificity or resolution. Here, we introduce interferometric photoactivated localization microscopy (iPALM), the combination of photoactivated localization microscopy with single-photon, simultaneous multiphase interferometry that provides sub-20-nm 3D protein localization with optimal molecular specificity. We demonstrate measurement of the 25-nm microtubule diameter, resolve the dorsal and ventral plasma membranes, and visualize the arrangement of integrin receptors within endoplasmic reticulum and adhesion complexes, 3D protein organization pr

SUBMITTER: Shtengel G 

PROVIDER: S-EPMC2637278 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature