Generation of a predicted protein database from EST data and application to iTRAQ analyses in grape (Vitis vinifera cv. Cabernet Sauvignon) berries at ripening initiation.

ABSTRACT:

Background

iTRAQ is a proteomics technique that uses isobaric tags for relative and absolute quantitation of tryptic peptides. In proteomics experiments, the detection and high confidence annotation of proteins and the significance of corresponding expression differences can depend on the quality and the species specificity of the tryptic peptide map database used for analysis of the data. For species for which finished genome sequence data are not available, identification of proteins relies on similarity to proteins from other species using comprehensive peptide map databases such as the MSDB.

Results

We were interested in characterizing ripening initiation ('veraison') in grape berries at the protein level in order to better define the molecular control of this important

SUBMITTER: Lucker J 

PROVIDER: S-EPMC2637896 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature