Gene expression profiling of neurochemically defined regions of the human brain by in situ hybridization-guided laser capture microdissection.

ABSTRACT: Laser capture microdissection (LCM) permits isolation of specific cell types and cell groups based upon morphology, anatomical landmarks and histochemical properties. This powerful technique can be used for region-specific dissection if the target structure is clearly delineated. However, it is difficult to visualize anatomical boundaries in an unstained specimen, while histological staining can complicate the microdissection process and compromise downstream processing and analysis. We now introduce a novel method in which in situ hybridization (ISH) signal is used to guide LCM on adjacent unstained sections to collect tissue from neurochemically defined regions of the human postmortem brain to minimize sample manipulation prior to analysis. This approach was validated in nuclei that prov

SUBMITTER: Bernard R 

PROVIDER: S-EPMC2637935 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature