Extracting transcription factor binding sites from unaligned gene sequences with statistical models.

ABSTRACT:

Background

Transcription factor binding sites (TFBSs) are crucial in the regulation of gene transcription. Recently, chromatin immunoprecipitation followed by cDNA microarray hybridization (ChIP-chip array) has been used to identify potential regulatory sequences, but the procedure can only map the probable protein-DNA interaction loci within 1-2 kb resolution. To find out the exact binding motifs, it is necessary to build a computational method to examine the ChIP-chip array binding sequences and search for possible motifs representing the transcription factor binding sites.

Results

We developed a program to find out accurate motif sites from a set of unaligned DNA sequences in the yeast genome. Compared with MDscan, the prediction results suggest that, overall, our algorit

SUBMITTER: Lu CC 

PROVIDER: S-EPMC2638147 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature