Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation.

ABSTRACT: Human cells are known to be more refractory than rodent cells against oncogenic transformation in vitro. To date, the molecular mechanisms underlying such resistance remain largely unknown. The combination of simian virus 40 early region and H-Ras V12 has been effective for transformation of rat embryo fibroblasts, but not for human cells. However, the additional ectopic expression of the telomerase catalytic subunit (hTERT) was reported to be capable of causing transformation of normal human cells. In this study, however, we demonstrate that the combined expression of the above-mentioned three genetic elements is not always sufficient to transform normal human diploid fibroblasts (HDF). Although the expression and function of these introduced genetic elements were essentially the same, am

SUBMITTER: Akagi T 

PROVIDER: S-EPMC263854 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature