Boron nutrition of tobacco BY-2 cells. V. oxidative damage is the major cause of cell death induced by boron deprivation.

ABSTRACT: Boron (B) is an essential micronutrient for vascular plants. However, it remains unclear how B deficiency leads to various metabolic disorders and cell death. To understand this mechanism, we analyzed the physiological changes in suspension-cultured tobacco (Nicotiana tabacum) BY-2 cells upon B deprivation. When 3-day-old cells were transferred to B-free medium, cell death was detectable as early as 12 h after treatment. The B-deprived cells accumulated more reactive oxygen species and lipid peroxides than control cells, and showed a slight but significant decrease in the cellular ascorbate pool. Supplementing the media with lipophilic antioxidants effectively suppressed the death of B-deprived cells, suggesting that the oxidative damage is the immediate and major cause of cell death under

SUBMITTER: Koshiba T 

PROVIDER: S-EPMC2638710 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature