Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm.

ABSTRACT: A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin gene cluster. The limited GluD-1 size polymorphism among the rice varieties is due to amino acid substitutions rather than to post-transcriptional modification. GluD-1 is maximally expressed in the starchy endosperm starting at 5 d after flowering (DAF) and increasing through 30 DAF, a major difference from the other glutelins which are primarily expressed in the subaleurone from 10-16 DAF. Only about 0.2 kb of the GluD-1 promoter was sufficient to confer inner starch

SUBMITTER: Kawakatsu T 

PROVIDER: S-EPMC2639022 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature