Characterization of gene expression profiles for different types of mast cells pooled from mouse stomach subregions by an RNA amplification method.

ABSTRACT:

Background

Mast cells (MCs) play pivotal roles in allergy and innate immunity and consist of heterogenous subclasses. However, the molecular basis determining the different characteristics of these multiple MC subclasses remains unclear.

Results

To approach this, we developed a method of RNA extraction/amplification for intact in vivo MCs pooled from frozen tissue sections, which enabled us to obtain the global gene expression pattern of pooled MCs belonging to the same subclass. MCs were isolated from the submucosa (sMCs) and mucosa (mMCs) of mouse stomach sections, respectively, 15 cells were pooled, and their RNA was extracted, amplified and subjected to microarray analysis. Known marker genes specific for mMCs and sMCs showed expected expression trends, indicating accura

SUBMITTER: Tsuchiya S 

PROVIDER: S-EPMC2639374 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature